Speciation of selenium iv and selenium vi using coupled ion chromatographyhydride generation atomic absorption spectrometry sabine goldberg, d. Pdf atomic absorption spectrometry in determination of. Flameless atomic absorption spectrophotometry of selenium. Double beam atomic absorption spectrophotometer model 209 equipped with a highintensity selenium hallow cathode lamp as the radiation. Determination of selenium in environmental samples using. Determination of selenium in blood and plant material by hydride generation and atomicabsorption spectroscopy o.
Uvphotochemical vapor generation of selenium for atomic absorption spectrometry. The limits of detection, defined as the concentration giving a signal equal to 3s of the blank, were 0. A procedure using open digestion followed by flame atomic absorption spectrometry is described for measuring the total selenium content of seenriched yeast. Pdf this paper reports determination of arsenic, antimony and selenium in different matrices using atomic absorption spectrometry, with atomisation in. Extended xray absorption fine structure spectroscopy of. Pdf determination of selenium by atomic absorption. Speciation of seleniumiv and seleniumvi using coupled. Atomic absorption spectroscopy for mercury, automated by. Selenium is measured in serum by atomic absorption spectrometry in a procedure based on the methods described by lewis et al. Optimization of instrument conditions for the analysis for mercury. When a beckman triple burner airhydrogen and a triplepass optical system are used. Selenium, atomic absorption spectrometric, hydride, automated. In this method of analysis for selenium in whole blood by flameless atomic absorption spectrophotometry, 1 ml of sample is first digested with perchloric and nitric acids. Pdf comparison of two methods using atomic absorption.
Speciation of seleniumiv and seleniumvi using coupled ion chromatographyhydride generation atomic absorption spectrometry sabine goldberg, d. Extended xray absorption fine structure spectroscopy of seleniumhyperdoped silicon bonna k. The determination of arsenic antimony, selenium and. A suitable detection technique in speciation studies for arsenic, selenium, antimony and mercury. Chemical vapor generation in conjunction with atomic absorption spectroscopy is one of the most powerful tools for the determination of trace elements in a variety of matrices. Atomic absorption spectroscopy introduction atomic absorption absorption spectroscopy aa or aas is one of the commonest instrumental methods for analyzing for metals and some metalloids. Absorption all modern perkinelmer atomic absorption instruments are capable of measuring both atomic absorption and atomic. In the case of selenium nanoparticles including those within cell biomass samples, xray fluorescence analysis xfa and electron energyloss spectroscopy eels can be used to detect the. Antimony, arsenic and selenium with hydride atomic. Pdf a hydride generation coupled to atomic absorption spectroscopy hgaas method is presented for the determination of selenium content in soil. Vanadium, selenium in some ghanaian mushrooms using atomic absorption spectrometry quarcoo a1, adotey g1 and gordon a1 1accra polytechnic, science laboratory technology department, box gp 561, accra.
Some atomic absorption hydride generation interelement interference reduction studies utilizing ion exchange resins. Interference of volatile hydrideforming elements in. Comparison of selenium determination in liver samples by. Determination of calcium, magnesium, and sodium by atomic. Selenium analysis and speciation in dietary supplements.
The metal analysis was done to detect zinc, cadmium. Hydride generation atomic absorption spectroscopy hgaas some elements, mostly metalloids like antimony, arsenic, selenium, and tellurium, are regularly measured by hgaas atomization technique. Selenium iv and tetrahydroborateiii borohydride were simultaneously retained on a strong anionexchange resin in a packed column. Validation of method for total selenium determination in. This work introduces, for the first time, sequential injection technique for hydride generation, highlights advantages of using programmable. Atomic absorption spectroscopy 4 spectroscopy icpoes, inductively coupled plasma mass spectrometry icpms, have left atomic absorption aa behind.
Cold vapor atomic spectroscopy was used as a model system, with detection of mercury by absorption at 254 nm and limit of detection of 9. An indirect atomicabsorption method for the determination. Optimization of instrument conditions for the analysis for. One of the rarest elements once known only for its toxicity plays a key role in all animal life essential component of the human diet. This paper reports determination of arsenic, antimony and selenium in different matrices using atomic absorption spectrometry, with atomisation in a graphite tube and with generation of hydrides. Determination of selenium in blood and plant material by. Analytical methods of selenium determination springerlink. Zaas graphite furnace atomic absorption spectroscopy with zeeman. Simplifies and speeds up analyses requiring graphite furnace atomic absorption spectroscopy complex sample preparation such as mercury. Flame atomic absorption spectrometry analytical methods 3 important warning warning eye, explosion and hearing hazard aspiration of perchloric acid and perchlorates into a nitrous oxide. Atomicabsorption spectroscopy should be ideal since it combines the advantages of both sensitivity and selectivity for many elements, but selenium is difficult to determine by this technique, because of the.
Selenium, atomic absorption spectrometric, hydride parameters and codes. Consult the spectrometer instruction manual for details on performing flame emission. Analytical methods for atomic absorption spectroscopy. Atomic absorption spectroscopy aas is another interesting type of spectroscopy it is specifically designed for the analysis of the metals and metalloids substances by definition, aas is a quantitative.
Water quality 2 determination of ca, mg, fe, and na by flame atomic spectrophotometry introduction atomic spectroscopy is one of the most widely used methods for quantitative elemental analysis. Quarcoo a, adotey g, gordon a 2014 detection and quantification of trace elements. Detection and quantification of trace elements chromium. Atomic absorption spectrometry in determination of arsenic, antimony and selenium in environmental samples september 2002 polish journal of environmental studies 115. Samples were analyzed using atomic absorption spectrometry to determine the concentration of dissolved metal. Direct quantitative speciation of selenium in selenium. Comparison of two methods using atomic absorption spectrometry for determination of selenium in food article pdf available in archives of industrial hygiene and toxicology 503. Atomic absorption spectrometer fitted with a hydride system, and a suitable radiation source for the determination of arsenic, antimony and selenium, for example electrodeless discharge lamp or a.
Hydride generation atomic absorption spectroscopy introduction atomic absorption absorption spectroscopy aas is one of the commonest instrumental methods for analyzing for metals and. Optimization and 75se radiotracer efficiency study. Skujins application note atomic absorption introduction the determination of selenium by atomic absorption spectroscopy 1,2 and also by atomic fluorescence spectroscopy 3 has been reported by several workers. The atomic absorption spectroscopy of selenium sciencedirect. Atomic absorption spectroscopy aas and atomic emission spectroscopy aes is a spectroanalytical procedure for the quantitative determination of chemical elements using the absorption of optical. Summary selenium can be determined in aqueous solution by atomic absorption spec troscopy at 1960, 2040,2063 or 2075 a. Inorganic interference study of automated arsenic and. Atomic absorption spectrometry in determi nation of. Determination of mercury in fish atomic absorption spectrophotometric method, method casam70. The chemical vapor generation atomic absorption spectrometry technique is.
Arsenic, antimony, selenium and tellurium certified atomic absorption standards ygml were obtained commercially fisher scientific co. Blood and tissue selenium determination by hydride. Atomic absorption spectrometry is a technique for determining the concentration of an element in a sample by measuring the absorption of electromagnetic radiation by the atomic vapour of the element. Simple method for determination of selenium in biological materials by flameless atomic absorption spectrometry using carbontube atomizer, talanta 25. The hydride generation method commonly used for atomic absorption spectrometry involves sending the hydrogen compound gas ash3 and h2se generated in a hydride vapor generator into a quartz. Pdf atomic absorption spectrometry in determination of arsenic. Main analytical techniques used for elemental analysis in. Atomic absorption application note author aaron hineman perkinelmer, inc. Skujins application note atomic absorption introduction the determination of selenium by atomic absorption spectroscopy 1,2 and. Pdf determination of selenium in environmental samples using.